Enzyme	 Source	                 Recognition Sequence  Cut

EcoRI   Escherichia coli	      5'GAATTC              5'---G     AATTC---3'                                             3'CTTAAG	         3'---CTTAA     G---5'

BamHI   Bacillus amyloliquefaciens 5'GGATCC              5'---G     GATCC---3'                                   3'CCTAGG              3'---CCTAG     G---5'

HindIII Haemophilus influenzae     5'AAGCTT              5'---A     AGCTT---3'                                   3'TTCGAA	         3'---TTCGA     A---5'

MstII   Microcoleus species        5'CCTNAGG                                   3'GGANTCC	

TaqI    Thermus aquaticus          5'TCGA                5'---T   CGA---3'                                   3'AGCT	         3'---AGC   T---5'

NotI    Nocardia otitidis          5'GCGGCCGC                                   3'CGCCGGCG	

HinfI   Haemophilus influenzae     5'GANTC                                   3'CTNAG

AluI*   Arthrobacter luteus        5'AGCT                5'---AG  CT---3'	                                   3'TCGA                3'---TC  GA---5'	* = đầu bằng